Neochamaejasmin A Induces Apoptosis in Human Osteosarcoma MG-63 Cells via Mitochondrial Dysfunction and Phosphatidylinositol 3 Kinase/Protein Kinase B/Glycogen Synthase Kinase-3-Beta Signaling Pathway

Neochamaejasmin A is an individual compound isolated from Stellera chamaejasme and exerts antitumor effects on several types of human cancer cells. However, the effect neochamaejasmin osteosarcoma cells has not yet been investigated. The main aim was to detect in vitro MG-63 were treated with different concentrations (20, 40, 80 μg/ml). Cell viability detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. apoptosis through cell morphology observation Annexin V/propidium iodide staining. Mitochondrial membrane potential measured rhodamine 123 expressions apoptosis-related proteins phosphatidylinositol 3 kinase/protein kinase B signaling analyzed western blotting. results showed that significantly inhibited proliferation induced MG- 63 triggered disorder mitochondrial potential, increased expression Bcl-2-associated X protein cleaved-caspase 3, decreased B-cell lymphoma 2, inactivation B/glycogen synthase kinase-3-beta pathway. These new findings may indicate be chosen as a candidate for chemotherapy.